PRODUCT
Home   /   PRODUCT   /   Proteinsimple
PRODUCT
LC
GC
Instrument
Vial / Filter / SPE
Biotage
Tedia
MJ Science
Proteinsimple
Proteinsimple
뷰페이지
제목 Peggy Sue 등록일 2018-12-10 18:40:54

Peggy Sue

 

Two ways to get separation, identification and quantitation

Peggy Sue™ lets you separate and analyze proteins by size or charge from 2-440 kDa either by immunoassay or total protein. Got small sample volumes or starting materials? No problem. She uses as little as 0.2 µg/µL of protein in just 5 µL of sample, and runs up to 96 samples in one experiment. Charge ahead and do more with Peggy Sue!

Peggy Sue

HOW CAN PEGGY SUE HELP YOU?

My sample is precious and I don't have enough for other methods

Sometimes you wish you could do more with your small and precious sample. Peggy Sue, our most frugal system can run up to 96 data points in one overnight run with as little as 5 µL of sample. And she can utilize that sample to the max by allowing you to interrogate those few microliters eight times, such as when analyzing an entire cell signaling pathway in a single run. Imagine all the data you can get from your sample!

I need more than size data

Sometimes a band from a Western is not telling you the entire story about your target of interest. Size-based separation will not be able to resolve phosphorylation isoforms and other post-translational modification. A charge assay (cIEF plus immunodetection) has the power to let you see beyond what's detected by a traditional Western. Peggy Sue gives you two ways to get separation, identification and quantitation of your sample.

I have too many samples and need higher throughput protein characterization

Our Simple Western systems offer the right throughput for your needs. Peggy Sue will process up to 96 data points per run in eight cycles of 12 data points each. Ideal for screening or applying the same assay to large number of samples. All the throughput you need with minimum hands-on time.

I am in a race to discover and I need my data faster

If analyzing 96 data points overnight is not impressive enough, maybe we should add that it includes data analysis. Set up and start your run in the afternoon and come back the next morning to find molecular weight (for size assay), charge isoform detection (charge assay) and signal quantification automatically calculated for each sample. If your time is as precious as your samples and you need results faster, we will help you win the race to discovery with Peggy Sue.

Click on the clock to see your time savings.

My current method is not reproducible

How reproducible will your current immunoassay be in analyzing 96 data points? With Peggy Sue you get intra- and inter-assay CV<20% for size and charge assays. How's that for reproducibility?

 

공지사항
Cat No. 제품명
004-800 Peggy Sue System
PS-N005 Peggy Sue System Installation and Training
004-820 Peggy Sue (w/o computer)
SM-S001 12- 230 kDa Peggy Sue or Sally Sue Separation Module
SM-S002 66-440 kDa Peggy Sue or Sally Sue Separation Module
SM-S003 2-40 kDa Peggy Sue or Sally Sue Separation Module
PS-T002 Peggy Sue or Sally Sue Size Training Kit
DM-004 Biotin Detection Module for Wes, Peggy Sue or Sally Sue
DM-001 Anti-Rabbit Detection Module for Wes, Peggy Sue or Sally Sue
DM-003 No secondary Detection Module for Wes, Peggy Sue or Sally Sue
DM-002 Anti-Mouse Detection Module for Wes, Peggy Sue or Sally Sue
DM-TP01 Total Protein Detection Module for Wes , Peggy Sue or Sally Sue
DM-005 Anti-Human IgG Detection Module for Wes, Peggy Sue or Sally Sue
DM-006 Anti-Goat Detection Module for Wes, Peggy Sue and Sally Sue
041-528 Sponge Pack for Peggy/Sally, Peggy Sue, Sally Sue, NanoPro 1000
042-512 Peggy Sue/Sally Sue Separation Matrix
042-513 Peggy Sue/Sally Sue Stacking Matrix
042-522 Peggy Sue or Sally Sue Peroxide
042-520 Peggy Sue or Sally Sue Wash Buffer
031-103 Peggy Sue or Sally Sue Repeater Pipette 0.5 mL Tips
041-606 Peggy or Sally Pipetter Guide
042-518 Peggy Sue or Sally Sue Matrix Removal Buffer
042-521 Peggy Sue or Sally Sue Luminol-S
031-102 Peggy Sue or Sally Sue Repeater Pipette
041-595 Instrument Cup (set of 3)
042-919 Peggy Sue/Sally Sue 66-440 kDa Running Buffer
042-918 Peggy Sue/Sally Sue 66-440 kDa Separation Matrix
PS-BL01-5 Total Protein Biotin Labeling Kit
SS700 Capillaries - Size Separation for Peggy Sue/Sally Sue (1 pack)
SS701 Capillaries - Size Separation for Peggy Sue/Sally Sue (5 pack)
043-226 Peggy Sue or Sally Sue 66-440 kDa Split Running Buffer 3 Kit
043-225 Peggy Sue or Sally Sue 12-230 kDa Split Running Buffer 2 Kit
043-120 Peggy Sue/Sally Sue Pipete Wash Solution Kit
043-328 Peggy Sue/Sally Sue 2-40 kDa Separation Matrix
043-326 Peggy Sue or Sally Sue 2-40 kDa Split Running Buffer 4 Kit
040-663 Assay Plate/Lid Kit for Peggy or NanoPro 1000, 5-Pack
042-191 10X System Control Primary Antibody-Mouse
042-206 Anti-Rabbit Secondary Antibody
042-414 Streptavidin-HRP
042-205 Anti-Mouse Secondary Antibody
042-486 Erk 1 Primary Antibody for Size Assays
042-488 HeLa Lysate Controls for Wes, Sally Sue and Peggy Sue
042-196 10X System Control Primary Antibody-Rabbit
PS-ST01-8 "Standard Pack 1 12-230 kDa"
042-203 Wes Antibody Diluent 2
PS-ST02-8 "Standard Pack 2 12-230"
PS-ST03-8 "Standard Pack 3 66-440 kDa"
PS-ST04-8 "Standard Pack 4 66-440 kDa"
042-976 Total Protein Streptavidin-HRP
PS-FL03-8 Fluorescent 5x Master Mix 3
PS-ST05-8 Standard Pack 5 2-40 kDa
043-379 Peroxide
PS-FL05-8 Fluorescent 5x Master Mix 5
PS-FL01-8 Fluorescent 5x Master Mix 1
043-311 Luminol-S
043-426 20X Anti-Rabbit HRP Conjugate
043-491-2 Anti-Human IgG Secondary HRP Antibody
043-524 Milk-free Antibody Diluent
043-459-2 Secondary Streptavidin-HRP
043-522-2 Anti-Goat Secondary HRP Antibody
CBS301 Peggy or Sally Core Kit
CBS-02-01 Peggy or Sally-Mouse (12-180 kDa) Size Separation Master Kit
CBS301RD Peggy or Sally Size Detection Kit-Rabbit
CBS-01-01 Peggy or Sally-Rabbit (12-180 kDa) Size Separation Master Kit
CBS301MD Peggy or Sally Size Detection Kit-Mouse
CBS301S1 Peggy or Sally Size Separation Kit
PS-T003 Peggy Sue System Charge Training Kit
041-127 Amplified Mouse Secondary Antibody Detection Kit
041-126 Amplified Rabbit Secondary Antibody Detection Kit
CBS2001 XDR Charge Separation Master Kit for Peggy Sue, Peggy or NanoPro 1000
CBS401 Lysis Kit - RIPA Buffer for Charge Assays
CBS403 Lysis Kit - Bicine/CHAPS Buffer for Charge Assays
CBS404 HeLa Lysate Controls Kit - Bicine/CHAPS Buffer for Charge Assays
040-031 pI Standard 7.0
040-032 pI Standard 7.3
040-309 Antibody Diluent
040-337 Anolyte Refill
040-338 Catholyte Refill (200 mL)
040-482 Aqueous Inhibitor Mix
040-474 Pan Erk Primary Antibody
040-483 RIPA Lysis Buffer
040-510 DMSO Inhibitor Mix
040-644 pI Standard Ladder 1
040-024 pI Standard 4.0
040-025 pI Standard 4.2
040-026 pI Standard 4.4
040-027 pI Standard 4.9
040-028 pI Standard 5.5
040-029 pI Standard 6.0
040-030 pI Standard 6.4
040-967 Premix G2, ampholyte-free separation gradient
040-973 Premix G2, Pharmalyte pH 5?8 separation gradient
040-968 Premix G2, pH 3?10 separation gradient
041-108 Wash Concentrate Refill
041-081 Goat-Anti-Rabbit HUX HRP Secondary Antibody
040-793 NanoPro Leveling Solution
041-036 pI Standard, 8.4 (50 uL)
040-969 Premix G2, pH 4?9 separation gradient
040-974 Premix G2, Servalyt pH 5?8 separation gradient
040-970 Premix G2, pH 4?7 (nested) separation gradient, pH 2-4 plug
040-477 Phospho-Erk 1/2 Primary Antibody
040-972 Premix G2, pH 5?8 (nested) separation gradient, pH 2?4 plug
040-645 pI Standard Ladder 2
040-646 pI Standard Ladder 3
040-649 Sample Diluent
040-652 Luminol for Charge Assays (Peggy Sue, Peggy or NanoPro 1000)
040-655 Goat-Anti-Mouse-HRP Secondary Antibody
040-656 Goat-Anti-Rabbit Secondary Antibody
040-764 Bicine/CHAPS Lysis Buffer and Sample Diluent
040-790 pI Standard 9.7
040-647 pI Standard Ladder 4
CBS700 Capillaries-Charge Separation for Peggy or NanoPro 1000
CBS701 Capillaries-Charge Separation for Peggy or NanoPro 1000 (5-Pack)
041-528
043-379
MR701 Capillaries- Size Separation for Peggy/Sally (5 pack)
041-270 Streptavidin-HRP (Simon, Sally, Peggy)
041-695 Single Use FL standard
041-248 Stacking Matrix
041-808 Antibody Diluent Plus
041-504 Peggy or Sally Matrix Removal Buffer
041-247 Separation Matrix
041-265 Simon, Sally, Peggy Sample Buffer
MR700 Capillaries- Size Separation for Peggy/Sally (1 pack)
041-586 Peggy/Sally Wash Buffer
041-505 Peggy or Sally Running Buffer
CBS201STD1 Simple Western Size Standard 1
001-531 Compass for Simple Western
리스트